Dr Jason Raymond
麻豆视频
My research project
Tracking plastic particles and co-contaminants in the food web at environmentally realistic concentrationsThis project would utilise a novel approach of radiolabelling plastic particles as well as their co-contaminants in order to detect and quantify them at the very low levels in which they exist in environmental systems. This will also provide the ability to track these plastic particles through complex marine environments and the food web into marine organisms.
Supervisors
This project would utilise a novel approach of radiolabelling plastic particles as well as their co-contaminants in order to detect and quantify them at the very low levels in which they exist in environmental systems. This will also provide the ability to track these plastic particles through complex marine environments and the food web into marine organisms.
Publications
This study presents, for the first time, the development and validation of a liquid chromatography and time鈥恛f鈥恌light mass鈥恠pectrometry (LC鈥揟OF鈥揗S) based assay to quantify mycophenolic acid (MPA) in patient samples as part of a routine therapeutic drug monitoring service. MPA was extracted from 50鈥壩糽 human plasma by protein precipitation, using sulindac as internal standard (IS). Separation was obtained on a Luna鈩 Omega polar C18 column kept at 40掳C. The mobile phase consisted of a mixture of acetonitrile鈥揹eionized water (50:50, v/v) with 0.1% formic acid at a flow rate of 350鈥壩糽/min. Analyte and IS were monitored on a TOF鈥揗S using a Jet鈥怱tream鈩 (electrospray) interface running in positive mode. Assay performance was evaluated by analysing patient plasma (N =鈥69) and external quality assessment (N鈥=鈥6) samples. The retention times were 2.66 and 2.18鈥塵in for MPA and IS, respectively. The lower limit of quantification of MPA was 0.1鈥壩糶/ml. The within鈥 and between鈥恆ssay reproducibility results ranged from 1.81 to 10.72%. Patient and external quality assessment sample results were comparable with those obtained previously by an in鈥恏ouse validated LC鈥揗S/MS method. This method showed satisfactory analytical performance for the determination of MPA in plasma over the calibration range of 0.1鈥15.0鈥壩糶/ml.